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Development of a Taqman real-time PCR assay for rapid detection and quantification of Vibrio tapetis in extrapallial fluids of clams ArchiMer
Bidault, Adeline; Richard, Gaelle G.; Le Bris, Cedric; Paillard, Christine.
The Gram-negative bacterium Vibrio tapetis is known as the causative agent of Brown Ring Disease (BRD) in the Manila clam Venerupis (=Ruditapes) philippinarum. This bivalve is the second most important species produced in aquaculture and has a high commercial value. In spite of the development of several molecular methods, no survey has been yet achieved to rapidly quantify the bacterium in the clam. In this study, we developed a Taqman real-time PCR assay targeting virB4 gene for accurate and quantitative identification of V. tapetis strains pathogenic to clams. Sensitivity and reproducibility of the method were assessed using either filtered sea water or extrapallial fluids of clam injected with the CECT4600(T) V. tapetis strain. Quantification curves of...
Tipo: Text Palavras-chave: Vibrio tapetis; VirB4 gene; Taqman real-time PCR; Molecular diagnostic; Venerupis philippinarum; Marine pathogen; Brown ring disease.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00308/41909/73194.pdf
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